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Microsampling Systems

microFAST Isotope – Dual Loop Syringe Loading & Injection

The microFAST Isotope combines the advantages of the microDX autosampler with single or dual loop FAST capabilities and syringe based sample loading/injection. The system accurately loads a loop and then smoothly injects the solution into a μFlow concentric nebulizer at defined rates from 5-1000 μL/min. The valve on the flow injection system selects from two discrete, parallel flow paths. This allows rapid switching between solutions with minimal dead volume between the valve and the nebulizer.

Features

  • Total sample consumption
  • Syringe load micro volumes (5-1000 µL)
  • Syringe inject at desired flow rate (5-1000 µL/min)
  • Dual loop system

Benefits

  • Reliably run small volume samples at 5-1000 µL/min
  • Dual loop for high throughput and reduced carryover
  • Stable injection profiles for precise isotope ratios
  • Combine with Apex 2 or Apex Omega for enhanced instrument sensitivity and low blanks

Features Diagram

Features Diagram

The microFAST Isotope utilizes a syringes to precisely load micro-volumes samples (as low as 5 µL) and inject them at any user defined flow rate. The figures below illustrate the capability to produce smooth injection profiles for 5 µL samples at 100 µL/min with excellent reproducibility (<2% RSD, n=10).

5 µL Injection at 100 µL/min

5 µL Injection at 100 µL/min

10 Replicates of 5 µL Injections at 100 µL/min

10 Replicates of 5 µL Injections at 100 µL/min

The microFAST Isotope precisely syringe injects micro-volume samples (5 µL) into the Apex Omega at 5 µL/min, 10 µL/min and 20 µL/min. The figures below plot signal profiles of approximately 60 sec, 30 sec and 15 sec for a 5 µl sample. Increased sensitivity with flow rate is a result of high sample transport efficiency using the Apex Omega. Smooth injection profiles for 5 µL samples at all flow rates, results in excellent reproducibility (<2% RSD, n=5).

5 µL Injections at 5, 10, and 20 µL/min with Apex Omega

5 µL Injections at 5, 10, and 20 µL/min with Apex Omega

5 µL Injections at 5, 10, and 20 µL/min with Apex Omega

5 µL Injections at 5, 10, and 20 µL/min with Apex Omega

Li isotope ratios are measured using the microFAST Isotope system. Syringe injecting 100 μL of sample at 20 μL /min provides nearly 5 minutes of steady state signal for isotope ratio analysis. External reproducibility is improved for the smallest absolute sample amounts (6ng) using low volume solution aliquots.

Syringe Loading & Injection

Syringe Loading & Injection

δ7Li (6.3 ng Li per quintuplicate)

δ7Li (6.3 ng Li per quintuplicate)

Minimizing the amount of Nd required to obtain desired precision (> 50 ppm) and throughput for precious oceanographic samples is illustrated in two figures. First, internal precision is for plotted for variable amounts of Nd injected at 100 µL/min for two minutes. Less than 50 ppm (2 RSD) internal precision is obtained for 2 ng samples of Nd. Repeat analysis of reference materials JNdi and La Jolla indicate that the external precision for 2 ng of Nd is similar to the 50 ppm internal precision and appropriate for oceanographic applications.

Sample Amount

Sample Amount

Reproducibility for 2 ng

Reproducibility for 2 ng

The microFAST Isotope and the Apex Omega provide high precision potassium isotope ratios with greatly reduced uptake and wash times, thereby improving frequency of sample standard bracketing.

NIST SRM 70b Against BSW

NIST SRM 70b Against BSW

Apex Omega and microFAST Isotope

Apex Omega and microFAST Isotope

Contact us for information on our microFAST Isotope Systems!

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